The demand for herbal remedies has surged since the coronavirus outbreak due to the side effects of conventional treatments. This has led to increased research into extracting phytochemicals from plants for biomedical applications. This research aims to evaluate the phytochemical content and antioxidant and antimicrobial properties of the medicinal plant P. pudica. Phytochemical evaluation revealed that hydroalcoholic (HYA) and isopropanol (IPA) extracts had superior compositions, with HYA showing the highest content of phenols, flavonoids, and saponins. The HYA extract exhibited robust antioxidant activity, particularly in DPPH scavenging (70.82% inhibition) and reducing power (1.57 absorbance). Antimicrobial tests showed lower MIC zones of inhibition for IPA and HYA extracts. The findings suggest that P. pudica stem extracts possess significant phytochemical properties, highlighting their potential as novel therapeutic sources.