Senna tora is a well-known plant of Fabaceae family. It is common weed found throughout the Indian subcontinent. Traditionally this plant is used for the treatment of leprosy, ringworm, skin and liver disorder. In the present study the antioxidant, antimicrobial and anticancer potential of the Senna tora extracts was evaluated. The ethanolic and aqueous extracts of the Senna tora leaves was prepared. The phytochemical constitution of both the extract was analysed by liquid chromatography mass spectroscopy (LCMS). The antioxidant potential was checked by DPPH assay and the antimicrobial activity was checked on different gram positive and gram-negative bacteria. MCF-7 cells were used to study the anticancer potential of Senna tora extract. Presence of various phytochemicals were noted in LCMS analysis. The ethanolic extract showed better antioxidant and antimicrobial results as compared to the aqueous extracts. The Senna tora extracts exhibited cytotoxic potential on MCF-7 cell lines and the IC50 was found to be 22.26 ± 1.23 μg/ml. From the above results it can be concluded that the phytochemicals (flavonoids, alkaloids, saponins, glycosides, fatty acids and etc) present in Senna tora leaves extracts are responsible for its antioxidant, antimicrobial and anticancer efficacy. This plant extracts can be used as a suitable drug candidate. However, further in-depth study is required to support this claim.