Medicinal plants can harbor an abundance of beneficial microorganisms such as yeast, fungus, and bacteria. Of them, bacteria are the
most promising sources of bioactive compounds, which have enormous implications for a variety of fields including business, agriculture,
and medicine. Cissus quadrangularis, an aromatic plant is widely known for its medicinal properties. The current study was carried out to
screen for the presence of Bacillus thuringiensis bacteria in the leaves of Cissus quadrangularis. Further investigation was done to explore
the contribution of the bacteria in the metabolite content were extracted using chloroform and carbon tetrachloride. The compounds
were identified using Gas Chromatography-Mass Spectrometry analysis. The analysis revealed the presence of significant metabolites like
α-pinene, 3-carene,o-cymene, Limonene, Eucalyptol, neral, , Isobornyl acetate, 2,4-Di-tert-butylphenol, Dibutyl phthalate, Benzaldehyde,
and Phthalic acid, di(2-propylpentyl) ester has medicinal value. This study confirms the presence of bacteria in the Cissus quadrangularis
leaves and the bacterial isolate from plants could be a potential source of pharmaceutical important compounds.