Author

Mahendra Singh1, Vimala Yerramilli1*, Laxman Nagar2

Abstract

Coccinia grandis L., a member of the family Cucurbitaceae, is a naturalized medicinal plant in India, commonly called as “ivy gourd.” It is a source of valuable natural compounds useful as anti-fungal, anti-hypersensitive, anti-hyperglycemic, anti-microbial, anti-ulcer, antioxidant, anti-inflammatory (for wound healing), anti-cancer, antipyretic, analgesic and hepatoprotective (against jaundice and hepatitis). Micropropagation can protect such plant wealth from vulnerability. However, the calli need to be tested and compared with parent plant for beneficial phytochemicals. Hence, the present studies were carried out for antioxidant efficacy assays and GC-MS-based phytochemical profiling using C. grandis leaf & stem calli, and corresponding explants. GC-MS analysis revealed 86 metabolites in the parent plant (stem, leaves) and callus (stem callus, leaf callus). Among the identified bioactive compounds, 9-Octadecenamide was recorded to be 85% in stem, 57.87% in stem callus and 64.36% in leaf callus and 10-Nonadecanol was 33.42% in leaves. Some valuable bioactive compounds of parent plant parts and callus, useful in the treatment of hepatic problems, could be identified as Hexadecanoic acid-methyl ester, 5-(Hydroxymethyl)-2-(dimethoxy methyl) furan; 1-Eicosanol; Gamma-sitosterol; Olean-12-en-3-ol, acetate, (3-beta)-; Benzene-propanoic acid,3,5-bis(91,1-dimethylethyl)-4-hydro; Heneicosane; Beta-Amyrin; 1-Chloroheptacosane; Neophytadiene; 1-Octadecanol; Canadine; Protopine; Isopropyl linoleate and Behenic alcohol.

Pages: 146-155

Doi Number 10.61289/jibs2023.28.09.1185

Keywords Coccinia grandis, DPPH, FRAP, GC-MS, TP, TF.