Author

Nishi Yadav and Renu Mishra

Abstract

Plants represent the reservoir of secondary metabolites, responsible for its medicinal and aromatic properties. This study is aimed to analyze leaf extract of Hyptis suaveolens qualitatively and quantitatively in terms of their phytoconstituents. The extracts were screened for the presence of various phytoconstituents using preliminary chemical tests for qualitative analysis. For quantitative analysis, total phenolic content was determined by Folin-Ciocalteu method, total flavonoid content by aluminum chloride assay and total alkaloid content was determined by spectrophotometric assay based on bromo cresol green reaction. The analysis revealed the presence of bioactive compounds such as alkaloids, glycosides, proteins, saponins, flavonoids, tannins and phenols in aqueous fraction. The ethanolic fraction contained glycoside, alkaloid, flavonoid, tannin, phenol, steroid and fats. In the methanolic fraction only alkaloid, steroid, saponin and proteins were present, whereas acetone extract contained glycoside, steroid, fats and resins. Maximum phytoconstituents were observed in the aqueous and ethanolic extract of the plant. In quantitative analysis among the extracts tested, ethanol extract of H. suaveolens leaves showed maximum amount of flavonoid content (2.98±0.52 mg/100mg), phenolic content (2.28±0.41 mg/100mg) and alkaloid content (0.77±0.33 mg/100mg) as compared to aqueous extract. In methanol extract only, alkaloid content (0.21±0.12 mg/100mg) was determined. Bioactive compounds contained in the leaf extracts included flavonoids, tannins, phenols, alkaloids and glycosides which suggests that constituents of the plant extracts could serve as a source of drugs useful in the treatment of various diseases.

Pages:334-340

Doi Number 10.5958/2455-7218.2022.00014.6

Keywords Atropine, gallic acid, Hyptis suaveolens, phytochemicals, quercetin