Author

Manish Thakur1and Tejinder Kaur2

Abstract

Medicinal herbs are extensively utilized in traditional medicine to treat a variety of infectious illnesses. Bacterial infections have a significant effect on public health. The purpose of this research was to ascertain the antibacterial activity of the medicinal plant Cuscuta reflexa that was collected from three distinct locations: Bilaspur (Himachal Pradesh, India), Chamba (Himachal Pradesh, India), and Jalandhar (Punjab, India). Three distinct sites were chosen for the plant sample in order to determine the effect of geographical location on its bactericidal potential. Methanol extracts of the plants were produced and evaluated for antibacterial activity against five distinct bacterial species, two of which were Gram-positive (Bacillus cereus and Staphylococcus aureus) and three of which were Gram-negative (Escherichia coli, Pseudomonas fluorescens and Pseudomonas aeruginosa). Different biochemical assays were used to conduct a preliminary qualitative phytochemical screening of plant extracts. The plant's antibacterial potential was determined using the well diffusion technique. All of the plant extracts demonstrated antibacterial action, with a particular emphasis on Gram-positive bacteria. Notably, preparations of C. reflexa from Bilaspur had the greatest antibacterial activity when compared to those from Chamba and Jalandhar. The examination of the Bilaspur sample using Fourier-transform infrared spectroscopy reveals the presence of a variety of functional groups. 

Pages:164-170

Doi Number 10.5958/2455-7218.2022.00005.5

Keywords Antibacterial, Bacteria, Cuscuta reflexa, Fourier-transform infrared spectroscopy, Phytochemical