E-ISSN No: 2455 - 7218

P-ISSN No: 0019 - 4468

Listed in UGC Care

Recuperative performance of 4-Cl-IAA and IAA in the fixation and assimilation of nitrogen in Vigna radiata


Barket Ali


Auxin analogues indole-3-acetic acid (IAA) and 4-chloroindole-3-acetic acid (4-Cl-IAA) were assessed for their comparative impact on nitrogenase, glutamine synthetase (GS), glutamate synthase (GOGAT), glutamine dehydrogenase (GDH), nitrate reductase (NR), and nitrite reductase (NiR) in mung bean cv T-44. The plants were grown in earthen pots and the seedlings were percolated with 10-8 or 10-6M of IAA or 4-Cl-IAA at 7 and 14 days after sowing (DAS) through roots. Leaf/nodule samples were collected at 30 days stage of growth to study different parameters pertaining to root, nodules and leaves. The treated plants exhibited an increase in different parameters; GS, GOGAT, GDH, NR, and NiR, both in nodules/roots and leaves when treated with different concentrations of IAA or 4-Cl-IAA. Leghemoglobin concentration, nitrogen and protein contents also showed a pattern synchronous with that of the enzymes of GS-GOGAT and NR-NiR systems. Overall 4-Cl-IAA was more effective then IAA.  However, 10-6M concentration IAA had an effect comparable to that of 10-8M of 4-Cl-IAA. Moreover, 4-Cl-IAA at 10-6M concentration was more effective than 10-8M dose of the analogue.



Doi Number 10.5958/2455-7218.2022.00023.7

Keywords Auxin, 4-Cl-IAA, Glutamine synthetase, Nitrate reductase, Nitrogenase