Author

KASIM MOOD, ABHITEJA SIRIKONDA, HEMALATHA KUDIKALA

AND VENKATESWAR RAO ALLINI

1Department of Biotechnology, Kakatiya University, Warangal-506009, Telangana, India

Email-Id: vrao.alleni@gmail.com Page Nu,ber: 341-348

Date of online publication: 31st December 2021

DOI:10.5958/2455-7218.2021.00040.1

Abstract

Lippia nodiflora is a fast-growing, small creeping perennial herb that belongs to the family Verbenaceae. L. nodiflora is used for ethnomedicinal and pharmacological applications like adenopathy, chronic indolent ulcers, knee joint pain, cold, fever, anticancer, antidiabetic, and gastrointestinal disorder. This study was aimed to optimize the in vitro seed germination protocol in medicinally important Lippia nodiflora, which has in vivo seed germination problem. Seeds were sterilized with different surface sterilizing agents at different time period. Sterilized seeds were inoculated on different strengths of MS basal medium with various levels of sucrose concentrations. By comparison of measured characteristic results, we found that the maximum percentage of seed germination was recorded as 85.26 ± 0.04 on ½ strength MS basal medium supplemented with 1% (w/v) sucrose when the seeds were surface sterilized with 0.1% HgCl for 5 minutes. We optimized the seed surface sterilization protocol and culture condition for the 2 first time that may be useful for regeneration, transformation, and conservation studies. 

Keywords: In vitro, Lippia nodiflora, MS media, Seed germination

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