Author

ARGHYA GHOSH

U.G. & P.G. Department of Botany, Darjeeling Government College, Darjeeling

Email: agbotphd@rediffmail.com

Abstract

The plant Curcuma longa L. is a pharmaceutically important one and almost all the parts of the plant especially rhizomes were reported to contain different types of terpenoid. Rhizomatous explants from three months old buds were cultured on Murashige and Skoog's (MS) medium supplemented with different concentrations of cytokinins and auxins. When the culture was performed in MS medium supplemented with 3 mg/L of 6-benzylamino purine, it yields 7.12±0.34bc numbers of shoots with an average shoot length of 8.32±0.47abd cm. The elongated shoots produced 6.12±0.73def roots with an average length of 4.93±0.30abc on MS medium supplemented with 2.5 mg/L of Indole 3 butyric acid (IBA) and showed 82% survivability after hardening. When the culture on 3 mg/L of 6-benzylamino purine was supplemented with 12 mg/L of the (Z)-7-methoxy-1, 5-dihydrobenzo [c] oxepine isolated from

Curcuma caesia Roxb., multiple numbers of shoots having an average shoot length of 3.29±0.17c cm were produced. The elongated shoots produced roots with an average length of 7.91±0.93ijk on MS medium supplemented with 2.5 mg/L of Indole 3 butyric acid (IBA) and 14 mg/L of (Z)-7-methoxy-1, 5-dihydrobenzo [c] oxepine. Hence, it can be concluded that although this terpenoid is a shoot growth retardant yet acts as elicitor for rooting.

 Keywords: Curcuma longa L., Curcuma caesia Roxb., (Z)-7-methoxy-1, 5-dihydrobenzo [c] oxepine (MDHBO), Plant growth regulator, 6-Benzylamino purine, Indole 3 butyric acid. 

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