Author

KARTHIKA PALANISAMY, VASANTH KRISHNAN AND DHANDAPANI GURUSAMY1

Molecular Biology Lab, Department of Botany, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India. 1Department of Botany, Kongunadu Arts and Science College (Autonomous), Bharathiar University, Coimbatore 641 029, Tamil

Abstract

The present research was performed to develop an indirect organogenesis protocol in Macrotyloma uniflorum via callus by using cotyledon and epicotyledon explants. Cotyledon explants were produce the best result on MS medium supplemented with 3.0 mg/L BAP. The maximum percentage (97%) of regeneration was obtained with the combination of BAP+GA (2.0+1.0 mg/L) from 3 cotyledon explants. Well established root system was developed from regenerated shoot lets in the medium combination with BAP+NAA (1.0+1.0 mg/L). Cotyledon explants were showed more responsible than epicotyledon in terms of callus induction and plant regeneration. This in vitro, developed plant lets were successfully acclimatized to the pots and established in the green house. This standardized in vitro regeneration system will facilitate further to development of a reliable procedure to accomplish genetic manipulation in this genus.

Keywords: Cotyledon, Callus induction, Epicotyledon, Indirect organogenesis, Regeneration

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