Anethum graveolens L. (Dill) is an aromatic annual herb of Apiaceae family, it is easily available and frequently grown in herb, vegetable and flower garden. It is used as a medicine because of its high therapeutic value. The objective of this study is to identify biologically  important functional groups present in aqueous, acetone, ethanol, methanol, hydroalcoholic, dichloromethane and chloroform of  Anethum graveolens L. leaves by using Fourier Transform Infrared Spectroscopy (FTIR). Absorption spectra obtained from Anethum graveolens L. leaves FTIR analysis were used to determine the prominent peaks, vibrational assignments, intensities and wave number. FTIR spectra of Anethum graveolens L. leaves indicates the presence of alcohol, aldehyde, carbohydrates, ester, phenol, ether and amine groups. The functional groups observed in the analysis were major component of secondary metabolites found in Anethum graveolens L. leaves. The determination of functional groups by FTIR helps to identify the potential bioactive constituents of Anethum graveolens L. leaves. The findings of present study suggest that the plant extracts have significant medicinal potential, due to the presence of various biologically important functional groups as a chemical composition. It will be eventually utilized to treat a variety of diseases.