MOLECULAR EVALUATION OF DIVERSITY IN SOME INDIAN BIGNONIACEAE
SEEMA CHAUHAN AND S.V.S. CHAUHAN
Academy of Life Sciences, 8/13 I Kaushalpur, Bye Pass Road, Agra-282005, India
Genetic diversity in some Bignoniaceae growing in India was evaluated on molecular basis. RAPD-PCR amplification of genomic isolates purified from 12 selected genotypes (species) of the family was performed with the same protocol and conditions for all the 20 primers. Out of 354 bands produced by 20 primers used, there were 353 polymorphic bands and the percentage of polymorphism with these primers was 99.71. The dendrogram constructed on the basis of 354 amplified products generated by random amplification of polymorphic DNA showed the separation of 12 genotypes into 11 major groups at 0.52 Jaccard's coefficient. Only one species Dolichandrone falcata growing at two different places was found in same cluster at Jaccard's coefficient. However, at 0.3 scale of Jaccard's coefficient, these genera segregated into 10 groups and Adenocalymma alliaceum and Tabebuia heterophylla formed another group. These results showed high degree of variability among all the genera of family Bignoniaceae.
Keywords: RAPD-PCR amplification, genomic isolates, Jaccard's coefficient, polymorphism[Download]