EFFECT OF DORMANCY BREAKING TREATMENTS ON GERMINATION , VIGOUR AND PHYSIO-BIOCHEMICAL CHANGES IN SEEDS OF RAUVOLFIA SERPENTINA (L.) Benth. ex Kurz–ENDANGERED MEDICINAL
NEHA SAYAL AND ANJU THAKUR
Department of Basic Sciences, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan, HP 173230, India
Rauvolfia serpentina (L.) Benth. ex Kurz is an endangered medicinal species which produces large proportion of non-viable seeds. In addition, the viable seeds show dormancy because of adverse influence of the stony endocarp, seed coat induced dormancy and nondeep physiological dormancy rendering the seed mediated propagation unsatisfactory . The present study indicates the effect of dormancy breaking treatments on germination and underlying physio-biochemical changes in Rauvolfia serpentina (L.) Benth. ex Kurz. Untreated seeds did not show germination, whereas, the maximum germination percentage (36.67%) was observed in seeds subjected to GA 200 ppm for 48 hrs, which was closely followed by GA 100 ppm for 48 hrs (28.89%), KNO 1% for 48 hrs (23.33%) 3 3 3 0 and chilling at 5 C for 4 weeks as well as thiourea 0.5% for 48 hrs (22.22%). Henceforth, above seed treatments can pave the way for breaking dormancy ; inducing gerinamtion and improving vigour of seeds of Rauvolfia serpentina which otherwise remained ungerminated. Physio-biochemical responses of seeds to these pre-sowing treatments have also been discussed.
Keywords: Germination percentage, Sarpagandha, Mean germination time, physio-biochemical responses , Seedling vigour index[Download]