QUALITATIVE AND QUANTITATIVE ANALYSIS OF THAUMATIN LIKE PROTEINS (TLPS) IN IN VIVO AND IN VITRO CULTURES OF GYMNEMA SYLVESTRE
SAURABHA B ZIMARE , GITANJALI JAVIR , JYOTI OTAGERI , 3 1 KALPANA PAI AND NUTAN P MALPATHAK 1 2 Department of Botany, Savitribai Phule Pune University, Pune- 411007, (MH), India; Naoroji Godrej Centre for Plant Research (NGCPR). C/o Lawkim Motors Division. Gate no. 431, Shindewadi, Post 3 Shirwal, Dist. Satara. Maharashtra, India; Department of Zoology, Savitribai Phule Pune niversity, Pune- 411007, (MH), India. Email:firstname.lastname@example.org
Plant produces different pathogenesis related proteins as a defence mechanism against pathogenic conditions and in adaptation to stressful environment. TLPs are a group of proteins from PR 5 category which have shown homology with thaumatin from Thaumatococcus danielliiand have shown antibacterial activity. Exploration of PR proteins from in vivo plant materials (leaves) and from in vitro (cell suspension and shoot) cultures of G. sylvestre using Western blot and ELISA techniques has been reported for the first time. Western blot analysis showed a prominent band at 22 kDa which correspond to the TLPs of Gymnema sylvestre which compared with standard TLPs. ELISA analysis showed higher quantity of TLPs (2.067 μg/3g) in in vivo plant materials as compared to shoot cultures (1.729 μg/ 3g) and cell suspension cultures (1.721 μg/3g).
Keywords- ELISA, G. sylvestre, SDS- PAGE, TLPs, Western blot[Download]