RAPID IN VITRO REGENERATION OF A MEDICINALLY IMPORTANT PLANT GYMNEMA SYLVESTRE R.BR. THROUGH NODAL EXPLANTS
M. PRASHANTI, K. KALPANA AND B. ARUNJYOTHI
, Department of Botany, Osmania University, Hyderabad, Telangana - 500007
1. Department of Genetics, Osmania University, Hyderabad, Telangana -500007
The present study deals with an efficient in vitro propagation of a medicinally important Gymnema sylvestre R.Br. known for its antidiabetic activity that is being used in folk, ayurvedic and homeopathic systems of medicine since centuries. Among various combinations of growth hormones tested, highest induction of multiple shoots were observed on MS medium supplemented with 2.0mg/l Adenine sulphate+1.0mg/l BAP. Rooting was optimized in half strength MS medium supplemented with 2.0 mg/l IBA. The present protocol employed in this study can be used for large scale production to meet the pharmaceutical demands for commercial purpose.
Key words: Gymnema sylvestre R.Br., in vitro regeneration, adenine sulphate, shoot regeneration, root formation[Download]