RAPID IN VITRO PROPAGATION OF MEDICINALLY IMPORTANT HEMIDESMUS INDICUS (L.) R.BR. THROUGH NODAL EXPLANTS
*M. PRASHANTI1 , K.KALPANA1 , P.SHIVAPRASAD2 , SWATHI
SINGH2 , A.ROJARANI2 AND K. JANARDHAN REDDY1
1. Department of Botany, Osmania University, Hyderabad, Telangana - 500007
2. Department of Genetics, Osmania University, Hyderabad, Telangana - 500007
The present study deals with a new and an efficient protocol for in vitro propagation of medicinally important Hemidesmus indicus (L.) R.Br. from nodal explants. Among different cytokinins with varied concentrations of adenine sulphate along with BAP showed the best response for multiple shoot induction. Multiple shoot regeneration was established on MS medium supplemented with 4.0 mg/l adenine sulphate and 2.0 mg/l BAP. The regenerated shoots developed roots on MS medium supplemented with 4.0 mg/l IAA. About 78% of in vitro grown plants were successfully acclimatized in the field without any phenotypic changes.
Keywords: Hemidesmus indicus (L.) R.Br, micropropagation, nodal explants, adenine sulphate, shoot multiplication, root induction.[Download]